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Two barley aleurone-specific promoters from genes encoding lipid
transfer protein (Ltp1) and chitinase (Chi26), respectively, were
transcriptionally fused to a b-glucuronidase gene (GUS). Tissue-specific
expression of these GUS chimeric constructs was examined in transgenic
rice. Histochemical analysis of individual transformants revealed
that GUS expression could be detected only in the grains of transgenic
rice containing each chimeric construct. GUS expression under the
control of the Chi26 promoter was specifically observed in the aleurone
layers of rice grains. In contrast, GUS gene coupled to the Ltp1
promoter was not expressed in the aleurone layers but expressed
only in embryo tissue. This distinct expression pattern of two barley
aleurone-specific promoters in transgenic rice grains implies that
the molecular mechanism underlying the aleurone-specific expression
from those promoters may be different.
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