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Plant Cell Rep, Vol. 20 (2002) pp. 842-847 Analysis of the rice endosperm-specific globulin promoter in
transformed rice cells. The rice globulin (Glb) promoter was analyzed for its ability to
control the expression of the human lysozyme gene in transgenic
rice grains. The expression of the human lysozyme gene under the
control of the (Glb) promoter reached 23% total soluble protein,
with an average of 13%. To search for important elements within
the Glb promoter, we analyzed both 5' and internal deletions of
the rice globulin promoter using a transient expression assay system.
The promoter activity was decreased along with the progressive 5'
deletions. Every internal deletion also showed a reduction in transcription
activity. Our analyses indicate that multiple promoter elements
dispersed throughout the 896-bp globulin promoter sequence contribute
to the strong Glb promoter activity. Ó 2002 Springer-Verlag.
All rights reserved.
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